Vibrio crassostreae sp. nov., isolated from the haemolymph of oysters (Crassostrea gigas).
نویسندگان
چکیده
Polyphasic analysis of five new Vibrio isolates originating from the haemolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. gyrB phylogenetic analysis, fluorescent amplified-fragment length polymorphism (FAFLP) fingerprinting and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed to accommodate these isolates in a novel species, Vibrio crassostreae sp. nov. (type strain LGP 7(T)=LMG 22240(T)=CIP 108327(T)). Phenotypic and chemotaxonomic features that differentiate V. crassostreae from other known Vibrio species include arginine dihydrolase, utilization and fermentation of various carbon sources, beta-galactosidase activity, NO(2) production and the presence of the fatty acids 14 : 0 iso and 16 : 0 iso.
منابع مشابه
Vibrio gigantis sp. nov., isolated from the haemolymph of cultured oysters (Crassostrea gigas).
Polyphasic analysis of four new Vibrio isolates originating from the haemolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. Phylogenetic analysis based on DNA gyrase subunit B (gyrB), RNA polymerase sigma70 factor (rpoD), replication origin-binding protein (rctB) and transmembrane regulator...
متن کاملNocardia crassostreae sp. nov., the causal agent of nocardiosis in Pacific oysters.
Seven strains of bacteria were isolated from Pacific oysters, Crassostrea gigas, with a focal or systemic disease. The strains were aerobic, Gram-positive, acid-fast, produced a mycelium which fragmented into irregular rod-like elements, had a peptidoglycan containing meso-diaminopimelic acid, arabinose and galactose as major sugars, mycolic acids with 46-58 carbon atoms and G + C-rich DNA. All...
متن کاملMajor role of the outer-membrane protein OmpU in the Vibrio splendidus/ Crassostrea gigas interaction
Vibrio splendidus LGP32 is an oyster pathogen associated with the summer mortality syndrome that dramatically affects the production of Crassostrea gigas oysters worldwide (Gay et al., 2004; Saulnier et al., 2010). We have recently shown that the outer membrane protein OmpU is required for the virulence of V. splendidus in oysters (Duperthuy et al., 2010). In order to better understand the role...
متن کاملStress and stress-induced neuroendocrine changes increase the susceptibility of juvenile oysters (Crassostrea gigas) to Vibrio splendidus.
Oysters are permanently exposed to various microbes, and their defense system is continuously solicited to prevent accumulation of invading and pathogenic organisms. Therefore, impairment of the animal's defense system usually results in mass mortalities in cultured oyster stocks or increased bacterial loads in food products intended for human consumption. In the present study, experiments were...
متن کاملElevated temperature method for recovery of Vibrio cholerae from oysters (Crassostrea gigas).
Of 222 Vibrio cholerae isolates from diverse clinical and environmental sources, 219 produced visible growth in alkaline peptone broth when incubated overnight at 42 degrees C. In field trials conducted to compare enrichment at incubation temperatures of 42 and 35 degrees C, significantly higher rates of isolation (P less than 0.05) and recovery (P less than 0.01) of V. cholerae from oysters we...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- International journal of systematic and evolutionary microbiology
دوره 54 Pt 6 شماره
صفحات -
تاریخ انتشار 2004